Enzymatic Synthesis of Oligosaccharides Containing Ga 16 . 4 Gal Non - Reducing End Using ys - Galactanase from PeniciMum citrinum
نویسندگان
چکیده
The transglycesylation reaction was done with a fi-ga)actanase from Penieillium eitrinum. The regioseiectivity in the transglycosylation reaction was studied using soy bean arabinogalactan as a donor and monoeT disaccharide derivatives containing fi-galactosyl residue as accepters. We also synthesized oligosaccharides eontaining Galfi1.4Gal sequence such 2s Galfi1.4GalPl.4G]c, Galfi1.4Galfi1. 3GlcNAc, Galll1-4GalBl.4GlcNAe, Galfi1-,4Galfil.6GlcNAc, and Ga161-.4Galfi1-3GalNAc for use in the total s}'nthesis of complex sugar chains.
منابع مشابه
l-Fucose-containing arabinogalactan-protein in radish leaves
The carbohydrate moieties of arabinogalactan-proteins (AGPs) have β-(1 → 3)-galactan backbones to which side chains of (1 → 6)-linked β-Gal residues are attached through O-6. Some of these side chains are further substituted with other sugars. We investigated the structure of L-Fuc-containing oligosaccharides released from the carbohydrate moieties of a radish leaf AGP by digestion with α-L-ara...
متن کاملCarbohydrate specificity of a galectin from chicken liver (CG-16).
Owing to the expression of more than one type of galectin in animal tissues, the delineation of the functions of individual members of this lectin family requires the precise definition of their carbohydrate specificities. Thus, the binding properties of chicken liver galectin (CG-16) to glycoproteins (gps) and Streptococcus pneumoniae type 14 polysaccharide were studied by the biotin/avidin-me...
متن کاملIn vivo targeting function of N-linked oligosaccharides with terminating galactose and N-acetylgalactosamine residues.
N-Linked biantennary, triantennary, and core fucosylated biantennary oligosaccharides were isolated from animal glycoproteins and derivatized at their reducing end with Boc-tyrosine. The terminal Gal residues were enzymatically removed and replaced with GalNAc. Tyrosinamide-oligosaccharides were radioiodinated and administered intravenously to mice. Pharmacokinetic and biodistribution studies r...
متن کاملEnzymatic synthesis of complex glycosaminotrioses and study of their molecular recognition by hevein domains.
Hevein, a protein found in Hevea brasiliensis, has a CRD domain, which is known to bind chitin and GlcNAc-containing oligosaccharides. By using NMR and molecular modeling as major tools we have demonstrated that trisaccharides containing GalNAc and ManNAc residues are also recognized by hevein domains. Thus far unknown trisaccharides GlcNAcbeta(1-->4)GlcNAcbeta(1-->4)ManNAc (1) and GalNAcbeta(1...
متن کاملStructural determination of neutral O-linked oligosaccharide alditols by negative ion LC-electrospray-MSn.
Neutral O-linked oligosaccharides released from the salivary mucin MUC5B were separated and detected by negative ion LC-MS and LC-MS(2). The resolution of the chromatography and the information obtained from collision induced dissociation of detected [M - H](-) ions were usually sufficient to identify the sequence of individual oligosaccharides, illustrated by the fact that 50 different oligosa...
متن کامل